Calponin 2 Antibody (OTI2B5) - Azide and BSA Free

Western Blot: Calponin 2 Antibody (OTI2B5) - Azide and BSA Free [NBP2-70421] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calponin 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calponin 2.

Immunocytochemistry/Immunofluorescence: Calponin 2 Antibody (OTI2B5) - Azide and BSA Free [NBP2-70421] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Calponin 2.

Immunohistochemistry: Calponin 2 Antibody (OTI2B5) - Azide and BSA Free [NBP2-70421] - Staining of paraffin-embedded Human lymphoma tissue using anti-Calponin 2 mouse monoclonal antibody.

Western Blot: Calponin 2 Antibody (OTI2B5) - Azide and BSA Free [NBP2-70421] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Calponin 2 monoclonal antibody.

Immunohistochemistry: Calponin 2 Antibody (OTI2B5) - Azide and BSA Free [NBP2-70421] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Calponin 2 mouse monoclonal antibody.

Immunohistochemistry: Calponin 2 Antibody (OTI2B5) - Azide and BSA Free [NBP2-70421] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Calponin 2 mouse monoclonal antibody.

Immunohistochemistry: Calponin 2 Antibody (OTI2B5) - Azide and BSA Free [NBP2-70421] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Calponin 2 mouse monoclonal antibody.

Immunohistochemistry: Calponin 2 Antibody (OTI2B5) - Azide and BSA Free [NBP2-70421] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Calponin 2 mouse monoclonal antibody.

Immunohistochemistry: Calponin 2 Antibody (OTI2B5) - Azide and BSA Free [NBP2-70421] - Staining of paraffin-embedded Human tonsil using anti-Calponin 2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI2B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Calponin 2 Antibody (OTI2B5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Calponin 2 Antibody (OTI2B5) - Azide and BSA Free (NBP2-01325) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CNN2(NP_004359) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 33.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Calponin 2 Antibody (OTI2B5) - Azide and BSA Free

• calponin 2
• Calponin H2, smooth muscle
• calponin-2
• Neutral calponin

Background

Calponin, a 34 kDa protein, regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an actin- and tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-actin and inhibition of the actin-activated MgATPase activity of phosphorylated myosin.Both properties are lost following phosphorylation (primarily at serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin), and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNN2