Calponin 1 Antibody (SPM169)

Immunocytochemistry/Immunofluorescence: Calponin 1 Antibody (SPM169) [NBP2-44480] - Analysis of PFA-fixed K562 cells labeling Calponin 1 Antibody (SPM169) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.

Immunohistochemistry-Paraffin: Calponin 1 Antibody (SPM169) [NBP2-44480] - Human Uterus stained with Calponin-1 Monoclonal Antibody (SPM169).

Flow Cytometry: Calponin 1 Antibody (SPM169) [NBP2-44480] - Analysis of PFA-fixed K562 cells using Calponin 1 Antibody (SPM169) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

• Reactivity: Hu, Rt
• Applications: Flow, ICC/IF, IHC, IHC-P, ICC/IF
• Clone: SPM169
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Calponin 1 Antibody (SPM169) Summary

• Immunogen: Crude human uterus extract
• Localization: Cytoplasmic
• Marker: Smooth Muscle Marker
• Specificity: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
• Application Notes: Hu-chromosome location: 19p13.2
  Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Calponin 1 Antibody (SPM169)

• Basic calponin
• Calponin 1
• calponin 1, basic, smooth muscle
• Calponin H1
• Calponin H1, smooth muscle
• calponins, basic
• CNN1
• Sm-Calp
• SMCC
• SMCCcalponin-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNN1
• Uniprot:
  • P51911(Human)