Calponin 1 Antibody (EP798Y)

Product Discontinued

Calponin 1 Antibody (EP798Y) Summary

• Immunogen: A synthetic peptide corresponding to residues near the C-term of human Calponin was used as an immunogen.
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CNN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-10000
• Application Notes: This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.)
• Control:

  Calponin 1 Overexpression Lysate
  Human Bladder Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Culture supernatant in buffered aqueous solution
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for Calponin 1 Antibody (EP798Y)

• Basic calponin
• Calponin 1
• calponin 1, basic, smooth muscle
• Calponin H1
• Calponin H1, smooth muscle
• calponins, basic
• CNN1
• Sm-Calp
• SMCC
• SMCCcalponin-1

Background

Calponin is a 34 kD polypeptide that interacts with actin, tropomyosin, and calmodulin. It is involved in smooth muscle contraction mechanism and is restricted exclusively to smooth muscle tissue. Anti-calponin has been found to be useful in differentiating benign sclerosing lesions of the breast from carcinoma. Calponin positivity has also been noted in malignant myoepithelioma and pleomorphic adenoma of salivary gland origin, as well as angiomatoid malignant fibrous histiocytoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calponin 1 Antibody (NB110-55650)(5)

Publications using NB110-55650

• Galli D, Carubbi C, Masselli E et al. PKC-epsilon is a negative regulator of PVAT-derived vessel formation. Exp. Cell Res. 2015-01-15 [PMID: 25433270] (WB, Mouse)
  
  Details:
  Rabbit anti-PKC-epsilon / PKC epsilon antibody used at 1:100 dilution for IP application on 500 ug of protein extract from cardiomyocyte negative fractions (CMnegF) of control animals/C, of animals injected with mutant PKC epsilon K522M /rPKCEm and of animals injected with PKC-Epsilon over-expressing vectors/rPKCE (Fig. 5D). Antibody also used for IHC-P on mouse heart vessels with DAB and IF based detection systems (Fig. 3A, 3B and 3D).
• Papke CL, Cao J, Kwartler CS et al. Smooth muscle hyperplasia due to loss of smooth muscle alpha-actin is driven by activation of focal adhesion kinase, altered p53 localization and increased levels of platelet-derived growth factor receptor-beta. Hum Mol Genet 2013-04-24 [PMID: 23591991] (WB, Mouse)
• Elia A, Charalambous F, Georgiades P. New phenotypic aspects of the decidual spiral artery wall during early post-implantation mouse pregnancy. Biochemical and Biophysical Research Communications. 2011-11-12 [PMID: 22100651]
• Jeyasuria P, Wetzel J, Bradley M et al. Progesterone-Regulated Caspase 3 Action in the Mouse May Play a Role in Uterine Quiescence During Pregnancy Through Fragmentation of Uterine Myocyte Contractile Proteins. Biol Reprod 2009:biolreprod.108.70425. [PMID: 19144964]
• Guo X, Oshima H, Kitmura T et al. Stromal fibroblasts activated by tumor cells promote angiogenesis in mouse gastric cancer. J Biol Chem. 2008-07-01 [PMID: 18495668]

FAQs for Calponin 1 Antibody (NB110-55650). (Showing 1 - 1 of 1 FAQs).

1. We ordered the antibody Calponin (EP798Y), catalog number NB110-55650. The isotype is defined IgG. Could you please provide me the exact sub-isotype?
   • This is a Rabbit monoclonal and has not been subtyped. Rabbit monoclonal antibodies do not have subtypes like mouse or human so a general anti-Rabbit IgG secondary would be the most appropriate for your assays.

Bioinformatics

• Gene Symbol: CNN1
• Entrez:
  • 1264(Human)
  • 12797(Mouse)
  • 65204(Rat)
• Uniprot:
  • P51911(Human)