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Calponin 1 Antibody (CNN1/832) [DyLight 405]

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications Flow, IHC, IHC-P
Clone
CNN1/832
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47757AF350
Alexa Fluor 405
NBP2-47757AF405
Alexa Fluor 488
NBP2-47757AF488
Alexa Fluor 532
NBP2-47757AF532
Alexa Fluor 594
NBP2-47757AF594
Alexa Fluor 647
NBP2-47757AF647
Alexa Fluor 700
NBP2-47757AF700
Alexa Fluor 750
NBP2-47757AF750
Allophycocyanin
NBP2-47757APC
Biotin
NBP2-47757B
DyLight 350
NBP2-47757UV
DyLight 488
NBP2-47757G
DyLight 550
NBP2-47757R
DyLight 594
NBP2-47757DL594
DyLight 650
NBP2-47757C
DyLight 680
NBP2-47757FR
DyLight 755
NBP2-47757IR
FITC
NBP2-47757F
HRP
NBP2-47757H
Janelia Fluor 549
NBP2-47757JF549
Janelia Fluor 646
NBP2-47757JF646
PE
NBP2-47757PE
PerCP
NBP2-47757PCP
Unconjugated
NBP2-44481-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44481-0.1mg
Azide and BSA Free
NBP2-47757-0.1mg
IHC-Prediluted
NBP2-48205
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Calponin 1 Antibody (CNN1/832) [DyLight 405] Summary

Immunogen
Recombinant human CNN1 protein
Localization
Cytoplasmic
Marker
Smooth Muscle Marker
Specificity
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CNN1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Calponin 1 Antibody (CNN1/832) [DyLight 405]

  • Basic calponin
  • Calponin 1
  • calponin 1, basic, smooth muscle
  • Calponin H1
  • Calponin H1, smooth muscle
  • calponins, basic
  • CNN1
  • Sm-Calp
  • SMCC
  • SMCCcalponin-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NB100-56173] - Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfectionMCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies. Image collected and cropped by Cite Ab from the following publication (A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma. Oncotarget (2011) licensed under a CC-BY licence.
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Transgelin/TAGLN/SM22
NB600-507
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47757AF350
Alexa Fluor 405 NBP2-47757AF405
Alexa Fluor 488 NBP2-47757AF488
Alexa Fluor 532 NBP2-47757AF532
Alexa Fluor 594 NBP2-47757AF594
Alexa Fluor 647 NBP2-47757AF647
Alexa Fluor 700 NBP2-47757AF700
Alexa Fluor 750 NBP2-47757AF750
Allophycocyanin NBP2-47757APC
Biotin NBP2-47757B
DyLight 350 NBP2-47757UV
DyLight 405 NBP2-47757V
DyLight 488 NBP2-47757G
DyLight 550 NBP2-47757R
DyLight 594 NBP2-47757DL594
DyLight 650 NBP2-47757C
DyLight 680 NBP2-47757FR
DyLight 755 NBP2-47757IR
FITC NBP2-47757F
HRP NBP2-47757H
Janelia Fluor 549 NBP2-47757JF549
Janelia Fluor 646 NBP2-47757JF646
PE NBP2-47757PE
PerCP NBP2-47757PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CNN1