Calpain 5 Antibody

Images

 
Western Blot: Calpain 5 Antibody [NBP1-32222] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 7. 5% SDS PAGE Calpain-5 antibody, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Calpain 5 Antibody [NBP1-32222] - Paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.47 mg/ml

Order Details

Calpain 5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Calpain 5. The exact sequence is proprietary.
Predicted Species
Mouse (93%), Rat (92%), Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPN5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.47 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Calpain 5 Antibody

  • calpain 5
  • Calpain htra-3
  • calpain-5
  • EC 3.4.22
  • EC 3.4.22.54
  • FLJ46245
  • HTRA3NCL3
  • nCL-3EC 3.4.22.-
  • New calpain 3

Background

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CAPN5