Calpain 12 Antibody

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Immunocytochemistry/ Immunofluorescence: Calpain 12 Antibody [NBP2-49079] - Staining of human cell line CACO-2 shows localization to nucleoplasm & focal adhesion sites.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Calpain 12 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GDEADFTLRVFSERRHTAVEIDDVISADLQSLQGPYLPLELGLEQLFQELAGEEEELNASQLQALLSIALEPARAHTSTPREIGLRT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPN12
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Calpain 12 Recombinant Protein Antigen (NBP2-49079PEP)

Reactivity Notes

Mouse (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Calpain 12 Antibody

  • calcium-activated neutral proteinase 12
  • calpain 12
  • calpain-12
  • CANP 12
  • MGC20576

Background

Calpains are a family of cytosolic calcium-activated cysteine proteases involved in a variety of cellular processesincluding apoptosis, cell division, modulation of integrin-cytoskeletal interactions, and synaptic plasticity (Dear etal., 2000 (PubMed 10964513)). CAPN12 belongs to the calpain large subunit family.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CAPN12