Calpain 11 Antibody

Images

 
Western Blot: Calpain 11 Antibody [NBP2-15673] - Sample (30 ug of whole cell lysate) A: NT2D1 B: Hela 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Calpain 11 Antibody [NBP2-15673] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using Calpain 11 antibody (Green) at 1:200 dilution. Alpha-tubulin filaments are ...read more
Immunohistochemistry-Paraffin: Calpain 11 Antibody [NBP2-15673] - Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Calpain 11 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Calpain 11. The exact sequence is proprietary.
Localization
Acrosome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPN11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Calpain 11 Antibody

  • Calcium-activated neutral proteinase 11
  • calpain 11
  • calpain11
  • calpain-11
  • CANP 11
  • EC 3.4.22
  • EC 3.4.22.-
  • EC 3.4.22.52
  • EC 3.4.22.54

Background

Calpains constitute a family of intracellular calcium-dependent cysteine proteases. There are eight members in this superfamily. They consist of a variable 80 kDa subunit and an invariant 30 kDa subunit. This calpain protein appears to have protease activity and calcium-binding ability. A similar mouse protein may play a functional role in spermatogenesis and in the regulation of calcium-dependent signal transduction events during meiosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CAPN11
Uniprot