Calpain 11 Antibody - BSA Free

Western Blot: Calpain 11 Antibody [NBP2-15673] - Sample (30 ug of whole cell lysate) A: NT2D1 B: Hela 7. 5% SDS PAGE gel, diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: Calpain 11 Antibody [NBP2-15673] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using Calpain 11 antibody (Green) at 1:200 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: Calpain 11 Antibody [NBP2-15673] - Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Calpain 11 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Calpain 11 Antibody - BSA Free (NBP2-15673) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Calpain 11. The exact sequence is proprietary.
• Localization: Acrosome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAPN11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Calpain 11 Antibody - BSA Free

• Calcium-activated neutral proteinase 11
• calpain 11
• calpain11
• calpain-11
• CANP 11
• EC 3.4.22
• EC 3.4.22.-
• EC 3.4.22.52
• EC 3.4.22.54

Background

Calpains constitute a family of intracellular calcium-dependent cysteine proteases. There are eight members in this superfamily. They consist of a variable 80 kDa subunit and an invariant 30 kDa subunit. This calpain protein appears to have protease activity and calcium-binding ability. A similar mouse protein may play a functional role in spermatogenesis and in the regulation of calcium-dependent signal transduction events during meiosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CAPN11
• Uniprot:
  • Q9UMQ6(Human)