Calpain 9 Antibody (3A6)

Western Blot: Calpain 9 Antibody (3A6) [H00010753-M02] - CAPN9 monoclonal antibody (M02), clone 3A6. Analysis of CAPN9 expression in human colon.

Immunohistochemistry-Paraffin: Calpain 9 Antibody (3A6) [H00010753-M02] - Analysis of monoclonal antibody to CAPN9 on formalin-fixed paraffin-embedded human liver. Antibody concentration 1 ug/ml.

ELISA: Calpain 9 Antibody (3A6) [H00010753-M02] - Detection limit for recombinant GST tagged CAPN9 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clone: 3A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Calpain 9 Antibody (3A6) Summary

• Immunogen: CAPN9 (NP_006606, 591 a.a. ~ 690 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYADEELQLDFDDFLNCLVRLENASRVFQALSTKNKEFIHLNINEFIHLTMNI
• Specificity: CAPN9 - calpain 9
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAPN9
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Calpain 9 Antibody (3A6)

• calpain 9 (nCL-4)
• calpain 9
• calpain-9
• Digestive tract-specific calpain
• EC 3.4.22
• EC 3.4.22.54
• GC36
• NCL4
• nCL-4EC 3.4.22.-
• New calpain 4
• novel calpain large subunit-4
• Protein CG36

Background

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calpain 9 Antibody (H00010753-M02)(4)

Publications using H00010753-M02

• Davis J, Martin SG, Patel PM et al. Low calpain-9 is associated with adverse disease-specific survival following endocrine therapy in breast cancer. BMC Cancer. 2014-12-23 [PMID: 25539577]
• Storr SJ, Pu X, Davis J et al. Expression of the calpain system is associated with poor clinical outcome in gastro-oesophageal adenocarcinomas. J Gastroenterol. 2013-01-19 [PMID: 23329366]
• Hata S, Abe M, Suzuki H et al. Calpain 8/nCL-2 and Calpain 9/nCL-4 Constitute an Active Protease Complex, G-Calpain, Involved in Gastric Mucosal Defense. PLoS Genet. 2010-07-29 [PMID: 20686710]
• Chen CJ, Nguyen T, Shively JE. Role of calpain-9 and PKC-delta in the apoptotic mechanism of lumen formation in CEACAM1 transfected breast epithelial cells. Exp Cell Res. 2009-11-01 [PMID: 19909740]

Bioinformatics

• Gene Symbol: CAPN9
• Entrez:
  • 10753(Human)
• OMIM:
  • 606401(Human)
• Uniprot:
  • NP_006606(Human)