Calnexin Antibody

Western Blot: Calnexin Antibody [NBP2-12724] - Western blot analysis of Rat Tissue lysates showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody (NBP2-12724). Load: 15 ugprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (NBP2-12724) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NBP2-12724] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin Polyclonal Antibody (NBP2-12724). Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (NBP2-12724) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Immunohistochemistry: Calnexin Antibody [NBP2-12724] - Immunohistochemistry analysis using Rabbit Anti-Calnexin Polyclonal Antibody (NBP2-12724). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (NBP2-12724) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT.

Western Blot: Calnexin Antibody [NBP2-12724] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody (NBP2-12724). Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (NBP2-12724) at 1:1000.

Product Discontinued

Calnexin Antibody Summary

• Immunogen: Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
• Localization: Endoplasmic Reticulum , Endoplasmic Reticulum Lumen , Melanosome , Cytoplasm
• Marker: Endoplasmic Reticulum Membrane Marker
• Specificity: Detects the C-terminal domain of Calnexin approx 90kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CANX
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:2000
• Application Notes: A 1:1000 dilution of Calnexin-CT Antibody was sufficient for detection of Calnexin in 10 ug of HeLa cell lysate by ECL immunoblot analysis.

Reactivity Notes

Based on homology, It is predicted to detect Human , Mouse , Rat , Bovine , Monkey , Chicken , Guinea Pig , Hamster , Porcine , Avian , Quail , Rabbit , Sheep , Drosophila and Xenopus

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Calnexin Antibody

• calnexin
• CNX
• IP90FLJ26570
• Major histocompatibility complex class I antigen-binding protein p88
• P90

Background

Certificate of Analysis, A 1: 1000 dilution was sufficient for detection of Calnexin in 10ug of HeLa cell lysate by ECL immunoblot analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CANX
• Entrez:
  • 403908(Canine)
  • 821(Human)
• Uniprot:
  • P24643(Canine)