Calnexin Antibody

Western Blot: Calnexin Antibody [NBP1-37774] - Staining of CaCo-2 (A) with antibody at 0.1 ug/mL and NIH3T3 (B) cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NBP1-37774] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-37774] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Calnexin Antibody [NBP1-37774] - )Staining of Human Cerebellum (A) and Colorectal cancer (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Calnexin Antibody Summary

• Description: Novus Biologicals Goat Calnexin Antibody (NBP1-37774) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Calnexin Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SKTPELNLDQFHDKT corresponding to internal region (near N-Terminus) according to NP_001737.1.
• Marker: Endoplasmic Reticulum Membrane Marker
• Specificity: Reported variants represent identical protein (NP_001019820.1, NP_001737.1).
• Predicted Species: Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CANX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 1 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Calnexin Antibody

• calnexin
• CNX
• IP90FLJ26570
• Major histocompatibility complex class I antigen-binding protein p88
• P90

Background

Calnexin, an abundant approx. 90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their Cterminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calnexin Antibody (NBP1-37774)(3)

Publications using NBP1-37774

• Dahlke E, Anan Y, Klie L et al. Megalin Orchestrates FcRn Endocytosis and Trafficking Cells 2022-12-22 [PMID: 36611847] (ICC/IF, Opossum)
• Golubinskaya V, Elvin J, Ebefors K et al. Bestrophin-3 is differently expressed in normal and injured mouse glomerular podocytes. Acta Physiol (Oxf). 2015-04-25 [PMID: 25912364] (IHC-P, Mouse)
• Rosser MF, Grove DE, Chen L, Cyr DM. Assembly and misassembly of cystic fibrosis transmembrane conductance regulator: folding defects caused by deletion of F508 occur before and after the calnexin-dependent association of membrane spanning domain (MSD) 1 and MSD2. Mol Biol Cell 2008-11-01 [PMID: 18716059]

Bioinformatics

• Gene Symbol: CANX
• Entrez:
  • 821(Human)
  • 12330(Mouse)
  • 29144(Rat)
• Uniprot:
  • P27824(Human)