Caldesmon/CALD1 Antibody (CALD1/820)

Images

 
Western Blot: Caldesmon/CALD1 Antibody (CALD1/820) [NBP2-44423] - Western Blot Analysis of human Ovary tissue using Caldesmon/CALD1 antibody (CALD1/820).
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (CALD1/820) [NBP2-44423] - Human Uterus stained with Caldesmon Monoclonal Antibody (CALD1/820).

Product Details

Summary
Reactivity Hu, Rt(-)Species Glossary
Applications WB, IHC
Clone
CALD1/820
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Caldesmon/CALD1 Antibody (CALD1/820) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47818)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
Recombinant full-length human Caldesmon/CALD1 protein (Uniprot: Q05682)
Localization
Cytoplasmic
Marker
Smooth Muscle Marker
Specificity
Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (12080kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This monoclonal antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CALD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25-0.5 ug/ml
  • Western Blot 2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Caldesmon/CALD1 Antibody (CALD1/820)

  • CAD
  • CALD1
  • caldesmon 1
  • Caldesmon
  • CDM
  • CDMH-CAD
  • HCAD
  • LCAD
  • L-CAD
  • MGC21352
  • NAG22

Background

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caldesmon/CALD1 Antibody (NBP2-44423) (0)

There are no publications for Caldesmon/CALD1 Antibody (NBP2-44423).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Caldesmon/CALD1 Antibody (NBP2-44423) (0)

There are no reviews for Caldesmon/CALD1 Antibody (NBP2-44423). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caldesmon/CALD1 Antibody (NBP2-44423) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Caldesmon/CALD1 Products

Research Areas for Caldesmon/CALD1 Antibody (NBP2-44423)

Find related products by research area.

Blogs on Caldesmon/CALD1

There are no specific blogs for Caldesmon/CALD1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Caldesmon/CALD1 Antibody (CALD1/820) and receive a gift card or discount.

Bioinformatics

Gene Symbol CALD1
Uniprot