Calcium Activated Nucleotidase 1/CANT1 Antibody (OTI7F8)

Western Blot: Calcium Activated Nucleotidase 1/CANT1 Antibody (7F8) [NBP2-46056] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calcium Activated Nucleotidase 1.

Immunohistochemistry: Calcium Activated Nucleotidase 1/CANT1 Antibody (7F8) [NBP2-46056] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: Calcium Activated Nucleotidase 1/CANT1 Antibody (7F8) [NBP2-46056] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI7F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Calcium Activated Nucleotidase 1/CANT1 Antibody (OTI7F8) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 63-401 of human CANT1 (NP_620148) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CANT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 44.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Calcium Activated Nucleotidase 1/CANT1 Antibody (OTI7F8)

• Apyrase homolog
• calcium activated nucleotidase 1
• CANT1
• DBQD
• EC 3.6.1.6
• Putative MAPK-activating protein PM09
• Putative NF-kappa-B-activating protein 107
• SCAN1
• SCAN-1Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase
• SHAPY
• SHAPYmicromelic dwarfism with vertebral and metaphyseal abnormalities and advancedcarpotarsal ossification
• soluble Ca-activated nucleotidase, isozyme 1
• soluble calcium-activated nucleotidase 1
• soluble calcium-activated nucleotidase SCAN-1

Background

Calcium-activated nucleotidase-1 (EC 3.6.1.6) is an extracellular protein that functions as a nucleotide tri- anddiphosphatase (Smith et al., 2002 (PubMed 12234496)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CANT1