Calcitonin Antibody (4901)

Product Discontinued

Calcitonin Antibody (4901) Summary

• Immunogen: Purified rat alpha-CGRP
• Specificity: Specific for Calcitonin Gene Related Peptide. Also recognizes rat beta-CGRP and human alpha-CGRP (10-fold less sensitivity compared to rat alpha-CGRP). Suggested positive controls are dorsal root ganglia, pancreas and gut.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALCA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
• Application Notes: This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.
• Theoretical MW: 0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Tested in Human and Rat. Expected reactivity to most mammals.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Calcitonin Antibody (4901)

• ATP2B2plasma membrane calcium ATPase
• ATPase, Ca++ transporting, plasma membrane 4
• CALC1
• CALCA
• Calcitonin
• DKFZp686G08106
• DKFZp686M088
• EC 3.6.3
• EC 3.6.3.8
• Katacalcin
• Matrix-remodeling-associated protein 1
• matrix-remodelling associated 1
• MXRA1
• PCT
• Plasma membrane calcium ATPase isoform 4
• Plasma membrane calcium pump isoform 4
• plasma membrane calcium pump
• plasma membrane calcium-transporting ATPase 4
• PMCA4b
• PMCA4PMCA4x
• sarcolemmal calcium pump

Background

Calcitonin (Katacalcin) is a 32 amino acids poly-peptide hormone that is commonly expressed in the parafollicular or C cells in the thyroid gland. It is also synthesized in a wide variety of other tissues, including the lung and intestinal tract (1). Calcitonin is a potent plasma calcium-lowering peptide; it decreases the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin staining is used in the identification of C cell proliferative abnormalities, such as C cell hyperplasia and invasive tumors (2). Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors (e.g. medullary thyroid carcinomas). Calcitonin activitiy might be exhibited in amyloid deposits within the tumor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calcitonin Antibody (NBP1-30051)(1)

Publication using NBP1-30051

• Boess F, Bertinetti-Lapatki C, Zoffmann S et al. Effect of GLP1R agonists taspoglutide and liraglutide on primary thyroid C-cells from rodent and man. J Mol Endocrinol 2013-04-12 [PMID: 23463748] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CALCA
• Uniprot:
  • P01256(Rat)