Calcineurin A Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Calcineurin A Antibody [NBP1-88218] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.

Analysis in human cerebral cortex and pancreas tissues using HPA012778 antibody. Corresponding PPP3CA RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218] - Staining of human prostate shows moderate positivity in plasma membrane in glandular cells

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218] - Staining of human testis shows weak membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Analysis in human cell line SCLC-21H.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Calcineurin A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Calcineurin A Antibody - BSA Free (NBP1-88218) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP3CA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Calcineurin A Protein (NBP1-88218PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Calcineurin A Antibody - BSA Free

• calcineurin A alpha
• Calcineurin A
• Calmodulin-dependent calcineurin A subunit alpha isoform
• CALN
• CALNA
• CALNAcatalytic subunit, alpha isoform
• CAM-PRP catalytic subunit
• CNA
• CNA1
• CNA1CALNA1
• EC 3.1.3.16
• PP2B
• PP3CA
• PPP2B
• PPP2BCCN1
• PPP3CA
• protein phosphatase 2B, catalytic subunit, alpha isoform
• protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)
• protein phosphatase 3, catalytic subunit, alpha isozyme
• serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Background

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP3CA