Calcineurin A Antibody

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Western Blot: Calcineurin A Antibody [NBP1-33041] - Non-transfected (-) and Calcineurin A-transfected (+) 293T whole cell extracts (30ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A ...read more
Immunocytochemistry/ Immunofluorescence: Calcineurin A Antibody [NBP1-33041] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcineurin A protein stained by Calcineurin A antibody diluted at ...read more
Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-33041] - Paraffin-embedded human breast carcinoma dilution: 1:500.
Western Blot: Calcineurin A Antibody [NBP1-33041] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Calcineurin A Antibody [NBP1-33041] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Calcineurin A Antibody [NBP1-33041] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody diluted at 1:500.
Western Blot: Calcineurin A Antibody [NBP1-33041] - Rat tissue extract (50ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Calcineurin A Antibody [NBP1-33041] - Analysis of paraformaldehyde-fixed HeLa, using NBP1-33041.
Immunohistochemistry: Calcineurin A Antibody [NBP1-33041] - Paraffin-embedded gastric carcinoma; antibody dilution 1:500.
Immunohistochemistry: Calcineurin A Antibody [NBP1-33041] - Paraffin-embedded colon carcinoma; antibody dilution 1:500.
Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-33041] - Paraffin-embedded HBL435 xenograft; antibody dilution 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Mk, Rb, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.33 mg/ml

Order Details

Calcineurin A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Calcineurin A. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Porcine (99%), Bovine (100%), Rabbit (100%), Zebrafish (93%), Canine (100%), Monkey (100%), Xenopus (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP3CA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%), Xenopus laevis (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.33 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Calcineurin A Antibody

  • calcineurin A alpha
  • Calcineurin A
  • Calmodulin-dependent calcineurin A subunit alpha isoform
  • CALN
  • CALNA
  • CALNAcatalytic subunit, alpha isoform
  • CAM-PRP catalytic subunit
  • CNA
  • CNA1
  • CNA1CALNA1
  • EC 3.1.3.16
  • PP2B
  • PP3CA
  • PPP2B
  • PPP2BCCN1
  • PPP3CA
  • protein phosphatase 2B, catalytic subunit, alpha isoform
  • protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)
  • protein phosphatase 3, catalytic subunit, alpha isozyme
  • serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on Calcineurin A.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

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Bioinformatics

Gene Symbol PPP3CA
Entrez