Calcineurin A Antibody

Images

 
Western Blot: Calcineurin A Antibody [NBP1-33041] - Non-transfected (-) and Calcineurin A-transfected (+) 293T whole cell extracts (30ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A ...read more
Immunocytochemistry/ Immunofluorescence: Calcineurin A Antibody [NBP1-33041] - DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcineurin A stained by Calcineurin A ...read more
Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-33041] - Paraffin-embedded human breast carcinoma dilution: 1:500.
Western Blot: Calcineurin A Antibody [NBP1-33041] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody diluted at 1:500.
Western Blot: Calcineurin A Antibody [NBP1-33041] - Rat tissue extract (50ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Rt, Po, Bv, Ca, Rb, RM, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Calcineurin A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Calcineurin A. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Porcine (99%), Bovine (100%), Rabbit (100%), Zebrafish (93%), Rhesus Macaque (100%), Canine (100%), Xenopus (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP3CA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Calcineurin A Antibody

  • calcineurin A alpha
  • Calcineurin A
  • Calmodulin-dependent calcineurin A subunit alpha isoform
  • CALN
  • CALNA
  • CALNAcatalytic subunit, alpha isoform
  • CAM-PRP catalytic subunit
  • CNA
  • CNA1
  • CNA1CALNA1
  • EC 3.1.3.16
  • PP2B
  • PP3CA
  • PPP2B
  • PPP2BCCN1
  • PPP3CA
  • protein phosphatase 2B, catalytic subunit, alpha isoform
  • protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)
  • protein phosphatase 3, catalytic subunit, alpha isozyme
  • serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Background

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Calcineurin A.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit.  ...  Read full blog post.

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Bioinformatics

Gene Symbol PPP3CA