Calcineurin A Antibody

Images

 
Western Blot: Calcineurin A Antibody [NB110-40553] - Detection of Human and Mouse PPP3CA by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. ...read more
Immunohistochemistry: Calcineurin A Antibody [NB110-40553] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PPP3CA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Calcineurin A Antibody [NB110-40553] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB110-40553 used for WB at ...read more
Immunohistochemistry-Paraffin: Calcineurin A Antibody [NB110-40553] - FFPE section of human prostate carcinoma. Antibody used at a dilution of 1:250.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

Calcineurin A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 470 and the C-terminus (residue 521) of human Protein Phosphatase 3, Catalytic Subunit, Alpha isoform using the numbering given in entry NP_000935.1 (GeneID 5530).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP3CA
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Calcineurin A Antibody

  • calcineurin A alpha
  • Calcineurin A
  • Calmodulin-dependent calcineurin A subunit alpha isoform
  • CALN
  • CALNA
  • CALNAcatalytic subunit, alpha isoform
  • CAM-PRP catalytic subunit
  • CNA
  • CNA1
  • CNA1CALNA1
  • EC 3.1.3.16
  • PP2B
  • PP3CA
  • PPP2B
  • PPP2BCCN1
  • PPP3CA
  • protein phosphatase 2B, catalytic subunit, alpha isoform
  • protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)
  • protein phosphatase 3, catalytic subunit, alpha isozyme
  • serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Background

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Blogs on Calcineurin A.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

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Bioinformatics

Gene Symbol PPP3CA
Entrez
Uniprot