Calcineurin A Antibody (S01-6F5)

Western Blot: Calcineurin A Antibody (S01-6F5) [NBP3-19843] - Western blot detection of Calcineurin A in Hela, A549, U2OS cell lysates using NBP3-19843 (1:1000 diluted). Predicted band size: 59kDa. Observed band size: 59kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S01-6F5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Calcineurin A Antibody (S01-6F5) Summary

• Description: Novus Biologicals Rabbit Calcineurin A Antibody (S01-6F5) (NBP3-19843) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Calcineurin A (Uniprot # Q08209)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PPP3CA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Calcineurin A Antibody (S01-6F5)

• calcineurin A alpha
• Calcineurin A
• Calmodulin-dependent calcineurin A subunit alpha isoform
• CALN
• CALNA
• CALNAcatalytic subunit, alpha isoform
• CAM-PRP catalytic subunit
• CNA
• CNA1
• CNA1CALNA1
• EC 3.1.3.16
• PP2B
• PP3CA
• PPP2B
• PPP2BCCN1
• PPP3CA
• protein phosphatase 2B, catalytic subunit, alpha isoform
• protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)
• protein phosphatase 3, catalytic subunit, alpha isozyme
• serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Background

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP3CA