Cadherin-4/R-Cadherin Antibody [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

Cadherin-4/R-Cadherin Antibody [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-4/R-Cadherin
  His21-Ala731
  Accession # P39038
• Specificity: Detects mouse Cadherin-4/R-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Cadherin-4/R-Cadherin and recombinant mouse (rm) Cadherin-2 is observed and less than 1% cross-reactivity wi
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cadherin-4/R-Cadherin Antibody [Alexa Fluor® 532]

• CAD4
• cadherin 4, type 1, preproprotein
• cadherin 4, type 1, R-cadherin (retinal)
• Cadherin4
• Cadherin-4
• CDH4
• FLJ22202
• FLJ40547
• MGC126700
• MGC138355
• RCAD
• R-CAD
• R-Cadherin
• Retinal cadherin

Background

Cadherin-4 (also Retinal/R-Cadherin) is a 120 kDa (100 kDa predicted) member of the classical cadherin family of molecules. It is expressed on mammary epithelium, olfactory bulb neurons, cerebellar granule cells, vascular smooth muscle, astrocytes and pancreatic beta -cells. Cadherin-4 forms both homodimers and heterodimers with N-cadherin. These complexes will oligomerize, providing sources for adhesion and migration. Cadherin-4 also binds to KLRG1 expressed on NK, T and Treg lymphocytes. Mouse cadherin-4 proprecursor is 893 amino acids (aa) in length. It contains a 146 aa propeptide (aa 21-166) coupled to a 746 amino acid (aa) type I transmembrane mature protein (aa 167-913). The mature region possesses a 565 aa extracellular domain (aa 167-731) plus a 160 aa cytoplasmic region (aa 753-913). Consistent with classical cadherins, cadherin-4 contains five cadherin repeats in the extracellular domain (aa 167-721). There is one potential splice form that shows a 44 aa substitution for aa 291-913. Over aa 21-731, mouse cadherin-4 shares 98% and 93% aa identity with rat and human cadherin-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.