CA125/MUC16 Antibody

ELISA: CA125/MUC16 Antibody [25450002]

Product Discontinued

CA125/MUC16 Antibody Summary

• Immunogen: This antibody is specific for the N Terminus Region of the target protein.
• Specificity: This product is specific for Human MUC16.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MUC16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-1:2000
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Western Blot and Immunohistochemistry-Paraffin.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for CA125/MUC16 Antibody

• CA125 ovarian cancer antigen
• CA125
• CA-125
• CA125MUC-16
• FLJ14303
• MUC16
• mucin 16, cell surface associated
• mucin-16
• Ovarian cancer-related tumor marker CA125
• Ovarian carcinoma antigen CA125

Background

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CA125/MUC16 Antibody (25450002)(2)

Publications using 25450002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: MUC16
• Entrez:
  • 94025(Human)
• Uniprot:
  • Q8WXI7()