CA125/MUC16 Antibody (EPR1020(2))

Product Discontinued

CA125/MUC16 Antibody (EPR1020(2)) Summary

• Immunogen: A synthetic peptide corresponding to residues in human CA 125 was used as an immunogen.
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MUC16
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Western Blot 1:1000-10000
• Theoretical MW: 2353 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for CA125/MUC16 Antibody (EPR1020(2))

• CA125 ovarian cancer antigen
• CA125
• CA-125
• CA125MUC-16
• FLJ14303
• MUC16
• mucin 16, cell surface associated
• mucin-16
• Ovarian cancer-related tumor marker CA125
• Ovarian carcinoma antigen CA125

Background

Carcinoma antigen 125 (CA 125) is a high molecular weight tumor antigen that is the basis for a widely used serum assay for the monitoring of patients with ovarian epithelial cancer (1). It is a heavily glycosylated mucin expressed on the surface of ovarian tumor cells and on human MUC16, a cell surface bound mucin that is also shed by proteolytic cleavage (2, 3). Furthermore, MUC16 is a potent inhibitor of natural killer cell mediated anti-tumor cytotoxic responses (3). Binding of CA125 to membrane-bound mesothelin mediates heterotypic cell adhesion, which may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CA125/MUC16 Antibody (NBP1-96619)(2)

Publications using NBP1-96619

• Fung K, Sharma SK, KeinAnen O et al. A Molecularly Targeted Intraoperative Near-Infrared Fluorescence Imaging Agent for High-Grade Serous Ovarian Cancer Mol. Pharm. 2020-07-16 [PMID: 32644804] (IF/IHC, Human)
• Gasparotto G, Costab JP, Costa PI et al. Electrochemical immunosensor based on ZnO nanorods-Au nanoparticles nanohybrids for ovarian cancer antigen CA-125 detection. Mater Sci Eng C Mater Biol Appl. 2. 2017-02-10 [PMID: 28482492]

Bioinformatics

• Gene Symbol: MUC16
• Entrez:
  • 94025(Human)
• Uniprot:
  • Q8WXI7()