Recombinant Human C8G His Protein

SDS-Page: Recombinant Human C8G Protein [NBP1-72476] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human C8G His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-202 of Human C8G
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQKPQRPRRP ASPISTIQPK ANFDAQQFAG TWLLVAVGSA CRFLQEQGHR AEATTLHVAP QGTAMAVSTF RKLDGICWQV RQLYGDTGVL GRFLLQARGA RGAVHVVVAE TDYQSFAVLY LERAGQLSVK LYARSLPVSD SVLSGFEQRV QEAHLTEDQI FYFPKYGFCE AADQFHVLDE VRR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: C8G
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 22.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human C8G His Protein

• C8C
• complement component 8, gamma polypeptide
• complement component C8 gamma chain
• MGC142186

Background

C8gamma, also known as C8G, is one of three polypeptides (along with C8 alpha and C8 beta) that constitutes C8, a component of the complement system. C8 participates in the formation of Membrane Attack Complex (MAC). Patients with deficiency in C8 are vulnerable to certain bacteria infection. C8gamma is a secreted protein that is able to bind retinol and belongs to the lipocalin family and calycin superfamily. It is synthesised in the liver, monocytes and fibroblast and functions to clear pathogens from an infected host. Recombinant human C8G protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: C8G
• Uniprot:
  • P07360(Human)