Rat C1q ELISA Kit (Colorimetric)

ELISA: Rat C1q ELISA Kit (Colorimetric) [NBP2-74988] - Samples were spiked with high concentrations of Rat C1q and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat C1q ELISA Kit (Colorimetric) [NBP2-74988] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Rat C1q ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat C1 in samples. No significant cross-reactivity or interference between Rat C1 and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.23%
• Intra-Assay: CV% < 4.82%
• Spike Recovery: 88-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: C1QA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat C1q ELISA Kit (Colorimetric)

• A chain
• complement component 1, q subcomponent, A chain
• complement component 1, q subcomponent, alpha polypeptide

Background

C1q encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for C1q ELISA Kit (NBP2-74988)(1)

Publication using NBP2-74988

• Lombardi M, Parolisi R, Scaroni F et al. Detrimental and protective action of microglial extracellular vesicles on myelin lesions: astrocyte involvement in remyelination failure Acta Neuropathol. 2019-07-30 [PMID: 31363836] (ELISA, Mouse)

FAQs for C1q ELISA Kit (NBP2-74988). (Showing 1 - 3 of 3 FAQ).

1. I am looking for polyclonal/monoclonal antibodies against canine C1q and complement-related. I was wondering if your prooduct does.
   • Please see the provided link below which will take you to our website and all of our C1Q antibodies that are available. Unfortunately we do not currently have an antibody that has been tested in dog but if that would be something you are interested in doing, I would like to invite you to review our Innovator's rewards program. Should you have any questions or concerns, please do not hesitate to contact me directly. C1Q antibodies: Innovators Rewards Program
2. For NBP2-74988, I would like to measure C1q in rat serum samples. I understand it is necessary to determine the optimal sample dilutions for my particular experiments myself, but if you have any information from when you validated rat serum samples, could you share the recommended dilution of normal rat serum samples?
   • We have validated this kit with rat serum, and the samples were diluted 40000-80000 fold (200-800ug/mL). 
3. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: C1QA