C1q Antibody

Flow Cytometry: C1q Antibody [NB100-64420] - Effects of SAP on C1q Binding to S. pneumoniae Measured Using Flow Cytometry. (A-C) Proportion of ST2 (A), ST4 (B), and ST23F (C) S. pneumoniae bacteria positive for C1q after incubation in 90 ug/ml human C1q with or without addition of 10 or 50 ug/ml hSAP. (D) Example of flow cytometry histograms of C1q binding to ST4 S. pneumoniae after incubation in 90 ug/ml human C1q with or without addition of 50 ug/ml hSAP. (E) Proportion of S. pneumoniae positive for C1q after incubation in human serum with (black shading) or without (grey shading) addition of 50 ug/ml hSAP, using C1q-depleted serum (white shading) as a negative control. (F) Example of flow cytometry histograms of C1q binding to ST2 S. pneumoniae after incubation in human serum with or without addition of 50 ug/ml hSAP.

Product Discontinued

C1q Antibody Summary

• Immunogen: Human C1q, purified from plasma
• Specificity: Recognizes human C1q, a sub-component of the C1 complement component.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: C1QA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunodiffusion
• Control:

  C1q Overexpression Lysate

Reactivity Notes

Use in Human reported in scientific literature (PMID:33808005).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Glycine Buffered Saline (pH 7.4), 0.01% Benzamidine, 0.1% EACA, 1 mM EDTA
• Preservative: 0.09% Sodium Azide
• Purity: IgG purified

Alternate Names for C1q Antibody

• A chain
• complement component 1, q subcomponent, A chain
• complement component 1, q subcomponent, alpha polypeptide

Background

C1q is a sub-component of the first complement component C1, which is formed by the interaction of C1q with the tetramer C1s-C1r-C1r-C1s. C1q is a member of a family of soluble collagen-like proteins and is composed of three basic subunits termed A, B and C (27,550, 25,200, 23,800 Da respectively) which associate in the proportion 6 A-B subunits (52, 750 Da) and 3 C-C subunits (47,600 Da), to form whole C1q (459,300 Da). C1q binds to IgG and IgM immune complexes, this activates C1r and C1s, which are also bound to C1q and so initiates the classical pathway of complement activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for C1q Antibody (NB100-64420)(4)

Publications using NB100-64420

• Wheeler E, Lenhart-Pendergrass P, Rysavy N et al. Divergent host innate immune response to the smooth-to-roughM. abscessusadaptation to chronic infection bioRxiv 2023-05-18 [PMID: 37293112] (WB, Bacteria)
• Lenhart-Pendergrass PM, Malcolm KC, Wheeler E et al. Deficient Complement Opsonization Impairs Mycobacterium avium Killing by Neutrophils in Cystic Fibrosis Microbiology Spectrum 2023-02-14 [PMID: 36651756] (control for neutralization, Human)
• Qian SJ, Huang QR, Chen RY et al. Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis Frontiers in immunology 2021-09-08 [PMID: 34566966]
• Fante M, Decking S, Bruss C et al. Heat-Inactivation of Human Serum Destroys C1 Inhibitor, Pro-motes Immune Complex Formation, and Improves Human T Cell Function International Journal of Molecular Sciences 2021-03-05 [PMID: 33808005] (control for neutralization, Human)

FAQs for C1q Antibody (NB100-64420). (Showing 1 - 1 of 1 FAQs).

1. I am looking for polyclonal/monoclonal antibodies against canine C1q and complement-related. I was wondering if your prooduct does.
   • Please see the provided link below which will take you to our website and all of our C1Q antibodies that are available. Unfortunately we do not currently have an antibody that has been tested in dog but if that would be something you are interested in doing, I would like to invite you to review our Innovator's rewards program. Should you have any questions or concerns, please do not hesitate to contact me directly. C1Q antibodies: Innovators Rewards Program

Bioinformatics

• Gene Symbol: C1QA
• Entrez:
  • 712(Human)