c-jun Antibody (320831) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for the indicated time. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse c-Jun Monoclonal Antibody (Catalog # MAB2670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for c-Jun was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

c-Jun was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse c-Jun Monoclonal Antibody (Catalog # MAB2670) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: 320831
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

c-jun Antibody (320831) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human c-Jun
  Met1-Phe331
  Accession # P05412
• Specificity: Detects human and mouse c-Jun in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: JUN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for c-jun Antibody (320831) [Unconjugated]

• Activator protein 1
• AP1
• AP-1
• cJun
• c-Jun
• enhancer-binding protein AP1
• Jun activation domain binding protein
• jun oncogene
• jun proto-oncogene
• JUN
• Proto-oncogene c-Jun
• transcription factor AP-1
• V-jun avian sarcoma virus 17 oncogene homologp39
• v-jun sarcoma virus 17 oncogene homolog (avian)
• v-jun sarcoma virus 17 oncogene homolog

Background

c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for c-jun Antibody (MAB2670)(1)

Publication using MAB2670

• Y Ichimaru, M Sano, I Kajiwara, T Tobe, H Yoshioka, K Hayashi, H Ijichi, S Miyairi Indirubin 3'-Oxime Inhibits Migration, Invasion, and Metastasis InVivo in Mice Bearing Spontaneously Occurring Pancreatic Cancer via Blocking the RAF/ERK, AKT, and SAPK/JNK Pathways Transl Oncol, 2019-10-28;12(12):1574-1582. 2019-10-28 [PMID: 31671317] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: JUN
• Uniprot:
  • P05412()