BTN2A2/Butyrophilin 2 Antibody (4B11C8) - BSA Free

Western Blot: BTN2A2/Butyrophilin 2 Antibody (4B11C8) [NBP2-61718] - Analysis using BTN2A2 mAb against HEK293 (1) and BTN2A2 (AA: extra 57-237)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: BTN2A2/Butyrophilin 2 Antibody (4B11C8) [NBP2-61718] - Analysis of Hela cells using BTN2A2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Flow Cytometry: BTN2A2/Butyrophilin 2 Antibody (4B11C8) [NBP2-61718] - Analysis of Hela cells using BTN2A2 mouse mAb (green) and negative control (red).

Western Blot: BTN2A2/Butyrophilin 2 Antibody (4B11C8) [NBP2-61718] - Analysis using BTN2A2 mAb against human BTN2A2 (AA: extra 57-237) recombinant protein. (Expected MW is 46.4 kDa)

Western Blot: BTN2A2/Butyrophilin 2 Antibody (4B11C8) [NBP2-61718] - Analysis using BTN2A2 mouse mAb against K562 (1) cell lysate.

ELISA: BTN2A2/Butyrophilin 2 Antibody (4B11C8) [NBP2-61718] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 4B11C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BTN2A2/Butyrophilin 2 Antibody (4B11C8) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human BTN2A2/Butyrophilin 2 (AA: extra 57-237) expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: BTN2A2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for BTN2A2/Butyrophilin 2 Antibody (4B11C8) - BSA Free

• BTF2
• BTF2BT2.2butyrophilin 2
• BTN2
• BTN2A2
• Butyrophilin 2
• butyrophilin subfamily 2 member A2
• butyrophilin, subfamily 2, member A2
• FLJ41908

Background

Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BTN2A2/Butyrophilin 2 Antibody (NBP2-61718)(1)

Publication using NBP2-61718

• Frech M, Danzer H, Uchil P et al. Butyrophilin 2a2 (Btn2a2) expression on thymic epithelial cells promotes central T cell tolerance and prevents autoimmune disease Journal of autoimmunity 2023-06-23 [PMID: 37356345] (FLOW, Human)

Bioinformatics

• Gene Symbol: BTN2A2