BTLA/CD272 Antibody (753131)

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Western blot shows lysates of A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse BTLA Monoclonal Antibody (Catalog # MAB7600) followed by HRP-conjugated Anti-Rat IgG Secondary ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
753131
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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BTLA/CD272 Antibody (753131) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BTLA
Met1-Pro176
Accession # Q7TSA3
Specificity
Detects mouse BTLA in ELISA and Western Blot.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
BTLA
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BTLA/CD272 Antibody (753131)

  • B and T lymphocyte associated
  • B and T lymphocyte attenuator
  • B- and T-lymphocyte attenuator
  • B- and T-lymphocyte-associated protein
  • BTLA
  • BTLA1
  • CD272 antigen
  • CD272
  • FLJ16065
  • MGC129743

Background

B- and T-lymphocyte attenuator (BTLA; CD272) is a 70 kDa, Ig-superfamily, type I transmembrane glycoprotein that is structurally similar to the CD28 family of T cell co-stimulatory or coinhibitory molecules (1‑3). Unlike CD28 family members, however, the BTLA extracellular Ig domain is an I-type rather than a V-type domain, and BTLA does not form homodimers (4). BTLA also differs from CD28 family members through the interaction of its Ig domain with the TNF superfamily member HVEM (herpesvirus entry mediator; TNFSF14) rather than with B7 family ligands (5). BTLA is a coinhibitory molecule expressed on T cells, B cells and, depending on the mouse strain, macrophages, dendritic and NK cells (6). Expression is low in naïve T cells and increased during antigen-specific induction of anergy. In B cells, BTLA is highest when cells are mature and naïve (6). BTLA apparently limits T cell numbers, since deletion of BTLA results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR crosslinking (7). The 305 amino acid (aa) BTLA contains a 29 aa signal sequence, a 153 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 102 aa cytoplasmic domain. There are two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail that mediate inhibitory signaling (8, 9). The binding of the BTLA to HVEM does not preclude additional binding of a mammalian stimulatory HVEM ligand, either LIGHT or lymphotoxin-alpha to the complex (4). At least three alleles varying by up to ten extracellular amino acids occur in different mouse strains (6). The ECD of C57BL/6 BTLA shows 51%, 77% and 40% aa identity to that of human, rat and canine BTLA, respectively. A splice variant lacking the Ig domain, termed BTLAs, has been reported (3).

  1. Murphy, K. M. et al. (2006) Nat. Rev. Immunol. 6:671.
  2. Croft, M. (2005) Trends Immunol. 26:292.
  3. Watanabe, N. et al. (2003) Nat. Immunol. 4:670.
  4. Compaan, D. M. et al. (2005) J. Biol. Chem. 280:39553.
  5. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
  6. Hurchla, M. A. et al. (2005) J. Immunol. 174:3377.
  7. Krieg, C. et al. (2007) Nat. Immunol. 8:162.
  8. Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.
  9. Chemnitz, J. M. et al. (2006) J. Immunol. 176:6603.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BTLA
Entrez
Uniprot