Recombinant Human BRMS1 T7 Protein

SDS-Page: BRMS1 Recombinant Protein [NBP2-51518] - 15% SDS Page (3 ug)

SDS-Page: BRMS1 Recombinant Protein [NBP2-51518] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human BRMS1 T7 Protein Summary

• Description: A denatured recombinant protein with a N-Terminal T7-tag and corresponding to the amino acids 1-246 of Human BRMS1
  
  

  Source: E.coli

  Amino Acid Sequence: MASMTGGQQM GRGSHMPVQP PSKDTEEMEA EGDSAAEMNG EEEESEEERS GSQTESEEES SEMDDEDYER RRSECVSEML DLEKQFSELK EKLFRERLSQ LRLRLEEVGA ERAPEYTEPL GGLQRSLKIR IQVAGIYKGF CLDVIRNKYE CELQGAKQHL ESEKLLLYDT LQGELQERIQ RLEEDRQSLD LSSEWWDDKL HARGSSRSWD SLPPSKRKKA PLVSGPYIVY MLQEIDILED WTAIKKARAA VSPQKRKSDG P

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: BRMS1
• Purity: >80%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 29.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >80%, by SDS-PAGE

Alternate Names for Recombinant Human BRMS1 T7 Protein

• breast cancer metastasis suppressor 1
• breast cancer metastasis-suppressor 1
• DKFZP564A063

Background

BRMS1 reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BRMS1