Brg1 Antibody

Images

 
Western Blot: Brg1 Antibody [NBP2-22234] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence ...read more
Immunohistochemistry: Brg1 Antibody [NBP2-22234] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- BRG1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry: Brg1 Antibody [NBP2-22234] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- BRG1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Proximity Ligation Assay: Brg1 Antibody [NBP2-22234] - Secondary -Conjugate duolink II PLA in Hela cells. goat anti-human SMARC44 (NBP2-22234) and rabbit anti-human CREB1 (NB100-87001) image merged from DAPI (2 ms) and ...read more
Proximity Ligation Assay: Brg1 Antibody [NBP2-22234] - The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin ...read more
Immunoprecipitation: Brg1 Antibody [NBP2-22234] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22234 used for IP at 6 ug/mg lysate. BRG1 was also immunoprecipitated by rabbit ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

Order Details

Brg1 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 50 and 100 of human BRM/SWI2-related Gene 1 using the numbering given in entry NP_003063.2 (GeneID 6597).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SMARCA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
  • Proximity Ligation Assay 1:1 000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Brg1 Antibody

  • ATP-dependent helicase SMARCA4
  • BAF190
  • BAF190A
  • brahma protein-like 1
  • Brg1
  • BRG1-associated factor 190A
  • BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
  • BRM/SWI2-related gene 1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ39786
  • global transcription activator homologous sequence
  • homeotic gene regulator
  • hSNF2b
  • Mitotic growth and transcription activator
  • nuclear protein GRB1
  • Protein brahma homolog 1
  • Protein BRG-1
  • RTPS2
  • SMARCA4
  • SNF2
  • SNF2B
  • SNF2-beta
  • SNF2L4
  • SNF2LB
  • SNF2-like 4
  • subfamily a, member 4
  • sucrose nonfermenting-like 4
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI2
  • transcription activator BRG1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SMARCA4
Entrez
Uniprot