Recombinant Human BRDG1/STAP-1 His Protein

SDS-Page: Recombinant Human BRDG1/STAP-1 Protein [NBP1-98975] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human BRDG1/STAP-1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-295 of Human BRDG1/STAP-1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMMAKKP PKPAPRRIFQ ERLKITALPL YFEGFLLIKR SGYREYEHYW TELRGTTLFF YTDKKSIIYV DKLDIVDLTC LTEQNSTEKN CAKFTLVLPK EEVQLKTENT ESGEEWRGFI LTVTELSVPQ NVSLLPGQVI KLHEVLEREK KRRIETEQST SVEKEKEPTE DYVDVLNPMP ACFYTVSRKE ATEMLQKNPS LGNMILRPGS DSRNYSITIR QEIDIPRIKH YKVMSVGQNY TIELEKPVTL PNLFSVIDYF VKETRGNLRP FICSTDENTG QEPSMEGRSE KLKKNPHIA

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: STAP1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 36.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human BRDG1/STAP-1 His Protein

• BCR downstream-signaling protein 1
• BRDG1
• BRDG1STAP-1BCR downstream signaling 1
• Docking protein BRDG1
• signal transducing adaptor family member 1
• signal-transducing adaptor protein 1
• signal-transducing adaptor protein-1
• STAP1
• Stem cell adaptor protein 1

Background

STAP1, also known as signal-transducing adaptor protein 1, appears to functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a positive feedback loop, increasing Tec activity. Recombinant human STAP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: STAP1