BRDG1/STAP-1 Overexpression Lysate

Images

 
Western Blot: BRDG1/STAP-1 Overexpression Lysate [NBL1-16514] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for STAP-1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

BRDG1/STAP-1 Overexpression Lysate Summary

Description

BRDG1/STAP-1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204854

Accession#: NM_012108

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
STAP1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
34.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for BRDG1/STAP-1 Overexpression Lysate

  • BCR downstream-signaling protein 1
  • BRDG1
  • BRDG1STAP-1BCR downstream signaling 1
  • Docking protein BRDG1
  • signal transducing adaptor family member 1
  • signal-transducing adaptor protein 1
  • signal-transducing adaptor protein-1
  • STAP1
  • Stem cell adaptor protein 1

Background

The protein encoded by this gene functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a postive feedback loop, increasing Tec activity. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2685
Species: Hu
Applications: IHC, Simple Western, WB
NB100-1343
Species: Hu
Applications: Flow, ICC/IF, IHC,  IHC-P, PEP-ELISA, WB
NB300-987
Species: Hu
Applications: IHC,  IHC-P, PEP-ELISA, WB
NBP2-48615
Species: Hu
Applications: IHC,  IHC-P, WB
NB100-56104
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr,  IHC-P, IP, WB
NBP1-78295
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC,  IHC-P, WB
NBP1-91228
Species: Hu, Rt
Applications: IHC,  IHC-P, WB
AF5129
Species: Hu
Applications: WB
NBP1-37000
Species: Hu
Applications: IHC,  IHC-P, PEP-ELISA, WB
AF3206
Species: Hu, Mu, Rt
Applications: ICC, Simple Western, WB
H00004068-M01
Species: Hu, Mu
Applications: ELISA, Flow, Func, WB
NB100-807
Species: Hu
Applications: ICC/IF, PEP-ELISA, WB
AF5414
Species: Hu
Applications: Simple Western, WB
AF5887
Species: Hu
Applications: IHC, WB
MAB3291
Species: Hu
Applications: IHC, Neut, WB
NBP1-32945
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, IP, WB
236-EG
Species: Hu
Applications: BA
AF2168
Species: Hu, Mu
Applications: ChIP, Simple Western, WB
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr,  IHC-P, IP, PLA, S-ELISA, Simple Western, WB
NBL1-16514
Species: Hu
Applications: WB

Publications for BRDG1/STAP-1 Lysate (NBL1-16514) (0)

There are no publications for BRDG1/STAP-1 Lysate (NBL1-16514).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for BRDG1/STAP-1 Lysate (NBL1-16514) (0)

There are no reviews for BRDG1/STAP-1 Lysate (NBL1-16514). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BRDG1/STAP-1 Lysate (NBL1-16514) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional BRDG1/STAP-1 Products

Research Areas for BRDG1/STAP-1 Lysate (NBL1-16514)

Find related products by research area.

Blogs on BRDG1/STAP-1

There are no specific blogs for BRDG1/STAP-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BRDG1/STAP-1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol STAP1