Brachyury Antibody

Product Discontinued

Brachyury Antibody Summary

• Immunogen: Synthetic peptide corresponding to an internal region of human Brachyury.
• Marker: Mesoderm Marker
• Specificity: Reacts with an internal sequence of the human Brachyury protein. Sequence is 80% identical and 86% positive in mouse and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TBXT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:1000-1:2000
• Application Notes: This antibody is useful for Western Blot 1:1000-1:2000, and ELISA The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 47.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Brachyury Overexpression Lysate

Reactivity Notes

Reacts with human. Expected from sequence similarity: mouse and rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Brachyury Antibody

• brachyury protein
• Brachyury
• MGC104817
• Protein T
• SAVA
• T brachyury (mouse) homolog
• T brachyury homolog
• T Brachyury Transcription Factor
• T, brachyury homolog (mouse)
• T-Box Transcription Factor T
• TBXT
• TF
• TFT

Background

The Brachyury protein is required for the proper formation and differentiation of posterior mesoderm and for axial development in all vertebrates. The Brachyury gene encodes a transcription factor that binds to a specific DNA element via its N-terminal region. A protein motif within the DNA-binding domain, the so-called T box, is highly conserved among T homologs from different species and also defines a broader family of T-box genes Brachyury functions during the earlier stages of gastrulation, which suggests that at least one of its early roles is to regulate the morphogenetic behaviour of nascent mesoderm by altering cell autonomous properties.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TBXT
• Entrez:
  • 6862(Human)
• Uniprot:
  • NP_003172(Human)