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Brachyury Antibody (JE44-11)

Images

 
Western Blot: Brachyury Antibody (JE44-11) [NBP2-76880] - Western blot analysis of Brachyury / Bry on SH-SY-5Y lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunohistochemistry: Brachyury Antibody (JE44-11) [NBP2-76880] - Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue using anti-Brachyury antibody. The section was pre-treated using heat ...read more
Flow Cytometry: Brachyury Antibody (JE44-11) [NBP2-76880] - Flow cytometric analysis of Brachyury was done on A549 cells. The cells were fixed, permeabilized and stained with Brachyury antibody at 1/100 dilution (red) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC-P, IP
Clone
JE44-11
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Brachyury Antibody (JE44-11) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within human Brachyury aa 260-435. (SwissProt: O15178 Human;SwissProt: P20293 Mouse)
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
T
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Brachyury Antibody (JE44-11)

  • brachyury protein
  • Brachyury
  • MGC104817
  • Protein T
  • SAVA
  • T brachyury (mouse) homolog
  • T brachyury homolog
  • T Brachyury Transcription Factor
  • T, brachyury homolog (mouse)
  • T-Box Transcription Factor T
  • TBXT
  • TF
  • TFT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol T