Recombinant Human Borealin His Protein

SDS-Page: Recombinant Human Borealin Protein [NBP1-72298] - 15% SDS-PAGE (3ug)

SDS-Page: Recombinant Human Borealin Protein [NBP1-72298] - 15% SDS-PAGE (3ug)

Product Discontinued

Recombinant Human Borealin His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 19-280 of Human Borealin
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRKLASFLKD FDREVEIRIK QIESDRQNLL KEVDNLYNIE ILRLPKALRE MNWLDYFALG GNKQALEEAA TADLDITEIN KLTAEAIQTP LKSAKTRKVI QVDEMIVEEE EEEENERKNL QTARVKRCPP SKKRTQSIQG KGKGKRSSRA NTVTPAVGRL EVSMVKPTPG LTPRFDSRVF KTPGLRTPAA GERIYNISGN GSPLADSKEI FLTVPVGGGE SLRLLASDLQ RHSIAQLDPE ALGNIKKLSN RLAQICSSIR THK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CDCA8
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 31.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 50% glycerol, 1 mM EDTA, 0.2 M NaCl
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Borealin His Protein

• BOR
• BOREALIN
• cell division cycle associated 8
• Cell division cycle-associated protein 8
• Dasra B
• DasraB
• dasra-B
• FLJ10468
• FLJ12042
• hDasra-B
• MESRGP
• PESCRG3
• Pluripotent embryonic stem cell-related gene 3 protein

Background

CDCA8, also known as Borealin, is a component of the chromosomal passenger complex (CPC). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, CDCA8 protein may be required to direct the CPC to centromeric DNA. Deletion of CDCA8 delays mitotic progression and causes kinetochore-spindle to mis-attach. Recombinant human CDCA8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CDCA8