Bora Antibody - BSA Free

Western Blot: Bora Antibody [NBP1-77013] - Jurkat cell lysate with Bora antibody at (A) 1 and (B) 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: Bora Antibody - BSA Free [NBP1-77013] - Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 u/mL.

Immunohistochemistry: Bora Antibody - BSA Free [NBP1-77013] - Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Bora Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Bora Antibody - BSA Free (NBP1-77013) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human Bora. The immunogen is located within amino acids 70 - 120 of Bora. Amino Acid Squence: RIDKDVEDKRQKAIEE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BORA
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  Bora Peptide (NBP1-77013PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Bora Antibody - BSA Free

• Aurora Borealis
• Bora
• C13orf34
• chromosome 13 open reading frame 34
• FLJ22624
• hsBora
• protein aurora borealis

Background

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BORA
• Uniprot:
  • Q6PGQ7(Human)