BOB1 Antibody (SP92)

• Reactivity: Hu
• Applications: IHC
• Clone: SP92
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

BOB1 Antibody (SP92) Summary

• Description: Novus Biologicals Rabbit BOB1 Antibody (SP92) (NBP3-45109) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide within Human BOB1 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary
• Specificity: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNAbinding activity. It recognizes the POU domains of OCT1 and OCT2.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: POU2AF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS (pH 7.4), Tween-20, 0.2% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for BOB1 Antibody (SP92)

• B-cell-specific coactivator OBF-1
• BOB-1
• OBF-1
• OBF1BOB1
• OCAB
• OCA-B
• OCT-binding factor 1
• POU class 2 associating factor 1
• POU domain class 2, associating factor 1
• POU domain class 2-associating factor 1
• POU domain, class 2, associating factor 1

Background

BOB1 is a novel 35kDa protein designated Bob1 (B cell Oct binding protein 1) is a lymphocyte specific transcription co activator protein. B cell specific transcription is mediated by octamer motifs found in immunoglobulin heavy and light chain gene promoters and in some immunoglobulin enhancers. These sites are bound by the B cell specific Oct1 and Oct2 transcription factors, and the B cell restricted co activator BOB.1/OBF.1. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POU2AF1