Mouse BNP ELISA Kit (Colorimetric)

ELISA: Mouse BNP ELISA Kit (Colorimetric) [NBP2-70011] - Samples were spiked with high concentrations of Mouse BNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse BNP ELISA Kit (Colorimetric) [NBP2-70011] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Mouse BNP ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse BNP in samples. No significant cross-reactivity or interference between Mouse BNP and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 5%
• Intra-Assay: CV% < 5.45%
• Spike Recovery: 88-108%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NPPB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse BNP ELISA Kit (Colorimetric)

• BNF
• BNP
• brain type natriuretic peptide
• Gamma-brain natriuretic peptide
• natriuretic peptide B
• natriuretic peptide precursor B
• natriuretic peptides B
• natriuretic protein
• NPPB

Background

Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides, see fig. 18). BNP is a 32 amino acid peptide with disulfide bond between the cystein residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for BNP ELISA Kit (NBP2-70011)(7)

Publications using NBP2-70011

• Li H, Chen D, Zhang X et al. Screening of an FDA-approved compound library identifies apigenin for the treatment of myocardial injury International Journal of Biological Sciences 2023-10-30 [PMID: 37928261] (ELISA)
• Rutledge C, Enriquez A, Redding K et al. Liraglutide Protects Against Diastolic Dysfunction and Improves Ventricular Protein Translation Cardiovascular drugs and therapy 2023-06-29 [PMID: 37382868] (ELISA)
• Du L, Sun X, Gong H et al. Single cell and lineage tracing studies reveal the impact of CD34+ cells on myocardial fibrosis during heart failure Stem cell research & therapy 2023-02-20 [PMID: 36805782] (ELISA)
• Jordan TJM Investigations in Comparative Animal Models of Inflammatory and Autoimmune Skin Disease. Thesis 2023-01-01 (ELISA)
• Feliz‐Mosquea Y, Wilson A, Cruz‐Diaz N et al. Dietary influences on chemotherapy sensitivity and cardiotoxicity modulated by IRE1 targeting in triple‐negative breast cancer in female mice Physiological Reports 2025-09-21 [PMID: 40976979]
• Bruszt K, Horvath O, Ordog K et Al. Cardiac effects of OPA1 protein promotion in a transgenic animal model PLoS One 2024-11-21 [PMID: 39570915] (ELISA)
• Wen C, Lan M, Tan X et al. GSK3B Exacerbates Myocardial Ischemia/Reperfusion Injury by Inhibiting Myc Oxidative Medicine and Cellular Longevity 2022-04-29 [PMID: 35528516] (ELISA, Mouse)

FAQs for BNP ELISA Kit (NBP2-70011). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NPPB