BNP Antibody (370129) [Unconjugated]

Recombinant human Pro-BNP protein was seriallydiluted 2-fold and captured by Mouse Anti-Human N-Terminal Pro-BNP MonoclonalAntibody (Catalog # MAB9538) coated on a Clear Polystyrene Microplate (Catalog #DY990).Rabbit Anti-Human N-Terminal Pro-BNP Monoclonal Antibody (Catalog #MAB36042)was biotinylated and incubated with the protein captured on the plate.Detection of the standard curve was achieved by incubating Streptavidin-HRP(Catalog # DY998)followed by Substrate Solution (Catalog # DY999)and stopping the enzymatic reaction with Stop Solution (Catalog #DY994).

Recombinant Human Pro-BNP protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Terminal Pro- BNP Monoclonal Antibody (Catalog # MAB9769) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human N-Terminal Pro BNP Monoclonal Antibody (Catalog # MAB9538) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 370129
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

BNP Antibody (370129) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human N-Terminal Pro-BNP
  His27-His134
  Accession # P16860
• Specificity: Detects human N-Terminal Pro-BNP in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BNP Antibody (370129) [Unconjugated]

• BNF
• BNP
• brain type natriuretic peptide
• Gamma-brain natriuretic peptide
• natriuretic peptide B
• natriuretic peptide precursor B
• natriuretic peptides B
• natriuretic protein
• NPPB

Background

Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro‑BNP (NT-proBNP) and mature BNP. Plasma NT-proBNP is a marker for congestive heart failure, while mature BNP (aa 103‑134) promotes vasodilation and fluid and sodium excretion. Human BNP precursor shares 29% and 51% aa sequence identity with mouse and porcine BNP precursor, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P16860()