Recombinant Human BMX Protein

Product Discontinued

Recombinant Human BMX Protein Summary

• Description: Recombinant protein for Human BMX
  
  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(P51813)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab60877)
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: BMX
• Purity: >80% SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 29 nmol phosphate incorporated into Poly Glu-Tyr substrate per minute per mg protein at 30C using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >80% SDS-PAGE

Alternate Names for Recombinant Human BMX Protein

• BMX non-receptor tyrosine kinase
• BMX
• Bone marrow tyrosine kinase gene in chromosome X protein
• EC 2.7.10
• EC 2.7.10.2
• Epithelial and endothelial tyrosine kinase
• ETK
• Etk/Bmx cytosolic tyrosine kinase
• ETKcytoplasmic tyrosine-protein kinase BMX
• NTK38 tyrosine kinase
• NTK38
• PSCTK2
• PSCTK3

Background

The BMX gene encodes a novel non receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells (1). Bmx cDNA comprises a long open reading frame of 675 amino acids, containing one SH3, one SH2 and one tyrosine kinase domain, which are about 70% identical with Btk, Itk and Tec and somewhat less with Txk tyrosine kinase sequences. The amino terminal sequences of these four tyrosine kinases are about 40% identical and each contains a so-called pleckstrin homology (PH) domain. The 2.7 kb Bmx mRNA is expressed in endothelial cells and several human tissues by Northern blotting and an 80 kD Bmx polypeptide was detected in human endothelial cells. The BMX gene is located in chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked agammaglobulinemia. Bmx, is found to induce activation of the Stat signaling pathway (3). Bmx induced the tyrosine phosphorylation and DNA binding activity of all the Stat factors tested, including Stat1, Stat3, and Stat5, both in mammalian and insect cells. Bmx also induced transcriptional activation of Stat1- and Stat5-dependent reporter genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BMX