BMP-8b Antibody (13E6) - BSA Free

Western Blot: BMP-8b Antibody (13E6) [NBP2-42693] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed at (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate

Immunocytochemistry/Immunofluorescence: BMP-8b Antibody (13E6) [NBP2-42693] - Analysis in Hela cells. The cell was stained with ATGA0332 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western Blot: BMP-8b Antibody (13E6) [NBP2-42693] - The recombinant protein (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with antibody at (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 13E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BMP-8b Antibody (13E6) - BSA Free Summary

• Description: Novus Biologicals Mouse BMP-8b Antibody (13E6) - BSA Free (NBP2-42693) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human BMP-8b (264-402aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BMP8B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for BMP-8b Antibody (13E6) - BSA Free

• BMP-8
• BMP-8b
• bone morphogenetic protein 8b
• MGC131757
• OP-3

Background

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bonegrowth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identifiedby an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development.In addition, the fact that this BMP is closely related to BMP5 and BMP7 has led to speculation of possible boneinductive activity. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BMP8B
• Uniprot:
  • NP_001711(Human)