BMP-15/GDF-9B Antibody [Alexa Fluor® 488]

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

BMP-15/GDF-9B Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant mouse BMP-15/GDF-9B
  Gln268-Arg392
  Accession # Q9Z0L4
• Specificity: Detects mouse and rat BMP-15/GDF-9B in Western blots and recombinant mouse in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) BMP‑15 is observed and less than 1% cross‑reactivity with rhBMP-3 a
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-15/GDF-9B Antibody [Alexa Fluor® 488]

• BMP15
• BMP-15
• bone morphogenetic protein 15
• GDF-9B
• Growth/differentiation factor 9B
• ODG2
• POF4

Background

BMP-15 (Bone morphogenetic protein 15; also GDF-9B) is a 24-26 kDa member of the TGF-beta superfamily of proteins. It is expressed by late primary follicle oocytes, where it promotes the transition of preantral granulosa cells to cumulus cells, and later the expansion of cumulus cells. Mouse BMP-15 proprecursor is a 50-55 kDa, 367 amino acid (aa) glycoprotein. It is proteolytically cleaved to generate a 40 kDa prosegment (aa 26-267) plus a 24 kDa, 124 aa mature region (aa 268-392) that may be phosphorylated (on Ser7 of the mature molecule) and/or glycosylated. Secreted BMP-15 does not occur as a mature homodimer, but it does exist as a mature monomer, an uncleaved proprecursor, or as a noncovalent heterodimer composed of a cleaved mature region and its prosegment. The heterodimer may also form an oligomer. The BMP-15 prosegment reportedly forms a noncovalent heterodimer with 20 kDa mature GDF-9. Mature mouse BMP-15 shares 70% and 91% aa identity with human and rat BMP-15, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.