Mouse BMP-1/PCP ELISA Kit (Colorimetric)

ELISA: Mouse BMP-1/PCP ELISA Kit (Colorimetric) [NBP2-69979] - Samples were spiked with high concentrations of Mouse BMP-1/PCP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse BMP-1/PCP ELISA Kit (Colorimetric) [NBP2-69979] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL

Mouse BMP-1/PCP ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse BMP-1 in samples. No significant cross-reactivity or interference between Mouse BMP-1 and analogues was observed.
• Standard Curve Range: 78.13 - 5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.66%
• Intra-Assay: CV% < 4.33%
• Spike Recovery: 87-107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BMP1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse BMP-1/PCP ELISA Kit (Colorimetric)

• BMP1
• BMP-1
• bone morphogenetic protein 1
• EC 3.4.24
• EC 3.4.24.19
• FLJ44432
• Mammalian tolloid protein
• mTld
• PCOLC
• PCP
• PCP2
• procollagen C-endopeptidase
• Procollagen C-proteinase
• TLD

Background

Bone morphogenetic protein 1 (BMP1) is a 986 amino acid member of the bone morphogenetic protein (BMP) family. It was initially discovered for its role to induce cartilage and bone formation. It is a metalloprotease that cleaves the C-terminus of procollagen I, II and III while other BMPs are part of the TGF beta superfamily. It may play a role in herniation, congestive heart failure, breast cancer, myelofibrosis with myeloid metaplasia and infection by HIV-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for BMP-1/PCP ELISA Kit (NBP2-69979)(2)

Publications using NBP2-69979

• Sharma RK, Kamble SH, Krishnan S et al. Involvement of lysyl oxidase in the pathogenesis of arterial stiffness in chronic kidney disease American journal of physiology. Renal physiology 2023-04-01 [PMID: 36825626] (ELISA)
• Vukicevic S, Colliva A, Kufner V et al. Bone morphogenetic protein 1.3 inhibition decreases scar formation and supports cardiomyocyte survival after myocardial infarction Nature communications 2022-01-10 [PMID: 35013172] (ELISA)

FAQs for BMP-1/PCP ELISA Kit (NBP2-69979). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BMP1