BMI-1 Antibody

Images

 
Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL ...read more
Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP2-24693] - Immunofluorescence of BMI1 in human oral squamous cell carcinoma (UPCI:SCC029B) cells. These cells are part of a large collection of HNSCC (head and ...read more
Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BMI-1 Antibody Summary

Immunogen
A portion of amino acids 270-320 of human BMI1 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BMI1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2.0-3.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml

Reactivity Notes

The amino acid sequence used is 100% homologous in human (isoforms 1 and 2), mouse, rat, dog and bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for BMI-1 Antibody

  • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
  • B lymphoma Mo-MLV insertion region 1 homolog
  • BMI1 polycomb ring finger oncogene
  • BMI1
  • BMI-1
  • FLVI2/BMI1
  • MGC12685
  • murine leukemia viral (bmi-1) oncogene homolog
  • PCGF4
  • polycomb complex protein BMI-1
  • polycomb group protein Bmi1
  • polycomb group ring finger 4
  • RING finger protein 51
  • RNF51
  • RNF51Polycomb group RING finger protein 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BMI-1 Antibody (NBP2-24693). (Showing 1 - 1 of 1 FAQ).

  1. What positive controls would you recommend for using this product for IHC-P on human tissues?
    • The positive control tissues that we would suggest are as follows: Normal brain cortex or hippocampus, Cervical lymph node, Prostate Cancer, Colorectal carcinoma, Lung tissues (normal or cancer)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BMI1
Uniprot