BMI-1 Antibody

Images

 
Western Blot: BMI-1 Antibody [NB100-87026] - Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Immunohistochemistry-Paraffin: BMI-1 Antibody [NB100-87026] - Human pancreatic islet cell carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).
Independent Antibodies: Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 on HeLa whole cell lysate using NB100-87026. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 ...read more
Biological Strategies: Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Ch, Fe, PmSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

BMI-1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 276 and 326 of human B lymphoma Mo-MLV insertion region 1 homolog using the numbering given in entry NP_005171.4 (GeneID 648).
Predicted Species
Primate (100%), Chicken (100%), Feline (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BMI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
BMI-1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-87026 in the following applications:

Publications
Read Publication using NB100-87026.

Reactivity Notes

Cat (100%), Orangutan (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BMI-1 Antibody

  • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
  • B lymphoma Mo-MLV insertion region 1 homolog
  • BMI1 polycomb ring finger oncogene
  • BMI1
  • BMI-1
  • FLVI2/BMI1
  • MGC12685
  • murine leukemia viral (bmi-1) oncogene homolog
  • PCGF4
  • polycomb complex protein BMI-1
  • polycomb group protein Bmi1
  • polycomb group ring finger 4
  • RING finger protein 51
  • RNF51
  • RNF51Polycomb group RING finger protein 4

Background

BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) is a core component of the polycomb group (PcG) multiprotein PRC1 complex which plays an important role in transcriptional repression of gene expression during development. BMI1 association with RING protein 1B is important to the E3 ligase activity of RING1B in the PRC1 complex. BMI1 is an oncogene whose overexpression is associated with many types of human tumors and is a stem-cell marker and regulator of stem cell self-renewal. Alternate names for BMI1 include polycomb complex protein BMI-1, polycomb group RING finger protein 4, RING finger protein 51, PCGF4, and RNF51.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Bv, Ch, Fe, Pm
Applications: WB, IHC, IHC-P, IP

Publications for BMI-1 Antibody (NB100-87026)(1)


Review for BMI-1 Antibody (NB100-87026) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB100-87026:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Other 07/29/2009
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Summary

ApplicationWestern Blot
Sample Testedfetal lung fibroblast HFL1, Sample Amount: 40 ug
SpeciesOther
LotA1

Blocking

Blocking DetailsBlocking Buffer: TTBS 5% milk, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: 5% milk TTBS, Incubation Time: overnight, Incubation Temp: 4 C

Details

Detection NotesDetection Method: HRP, Exposure Time: 5 minutes, Specific Bands: 40 kDa, Non-Specific Bands: 80 kDa

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for BMI-1 Antibody (NB100-87026) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for BMI-1 Antibody (NB100-87026)

Discover related pathways, diseases and genes to BMI-1 Antibody (NB100-87026). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for BMI-1 Antibody (NB100-87026)

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Blogs on BMI-1

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Recent Reviews

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Anonymous
07/29/2009
Application: WB
Species: Other

Bioinformatics

Gene Symbol BMI1
Entrez
Uniprot