Blooms Syndrome Protein Blm Antibody

Images

 
Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Detection of Human BLM by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity ...read more
Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Whole cell lysate from 293T cells (35 ug for WB; 775 ug for IP). Antibody: Affinity purified goat anti-BLM used at 0.5 ug/ml for WB and 3 ug/mg lysate ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human H2AX (NB100-383). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human phospho-BRCA1 [s1524] (NB100-200). ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human NONO (NB100-1556). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human FAN CD 2(NBP1-22971). Image merged ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human MSH2 (NB100-620). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human MRE11A (NB100-276). Image merged ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human MLH1 (NB100-197). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human p53 (NB200-171). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human phospho-CHK1 [s317] (NB100-275). Image ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human RPA70 (NB100-2204). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human SFPQ (NB100-61044). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human NBS1 (NB100-60654). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human RFC1 (NB600-961). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human ATM (NB100-678). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human phospho-53BP1 [S25] (NB100-1803). ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat ani-human BLM (NB100-324) and rabbit anti-human BLAP75 (NB100-1720). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate DuolinkII PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human TERF2 (NB100-2577). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anit-human BRCA1 (NB100-61617). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human phospho-BRCA1 [S1280] (NB100-224). ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human CTF18 (NBP1-03346). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human RAD50 (NB110-60483). Image merged from ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) I and rabbit anti-human BLM.. Image merged from DAPI (2ms) ...read more
Proximity Ligation Assay: Blooms Syndrome Protein Blm Antibody [NB100-324] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human 53BP1 (NB100-305). Image merged from ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, PLA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

Blooms Syndrome Protein Blm Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BLM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
NB 100-324 may be used for Western Blot and immunoprecipitation. Suggested working dilutions: * WB - 1:1,000-1:10,000 (ECL) IP - 1 to 4 ul/mg of lysate IHC / ICC - Not Determined *The investigator should determine the optimal working dilution for a specific application.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Blooms Syndrome Protein Blm Antibody

  • Bloom syndrome
  • Bloom syndrome, RecQ helicase-like
  • BSBloom syndrome protein
  • DNA helicase, RecQ-like type 2
  • EC 3.6.1
  • EC 3.6.4.12
  • MGC126616
  • MGC131618
  • RecQ protein-like 3
  • RecQ2
  • RECQ2MGC131620
  • RECQL3RECQL2

Background

The Bloom's syndrome gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DexH-box-containing DNA and RNA helicases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BLM
Entrez
Uniprot