BLNK Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

BLNK Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human BLNK
  Gly226-Ser456
  Accession # Q8WV28
• Specificity: Detects endogenous human BLNK in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BLNK Antibody [Alexa Fluor® 488]

• AGM4
• B cell linker protein
• BASH
• B-cell adapter containing a SH2 domain protein
• B-cell adapter containing a Src homology 2 domain protein
• B-cell linker protein
• B-cell linker
• BLNK
• BLNK-s
• Cytoplasmic adapter protein
• Ly57
• MGC111051
• SLP65
• SLP-65BLNK-S
• SLP65Ly57
• Src homology 2 domain-containing leukocyte protein of 65 kDa

Background

BLNK (B cell linker protein; also SLP-65 and BASH) is a 50 kDa (predicted) cytoplasmic protein that runs anomalously at 65‑70 kDa in SDS‑PAGE. It is a B cell adapter molecule that, following phosphorylation by Syk, serves as a scaffold for assembly of macromolecular complexes that involve Grb2, Vav and PLC gamma . Tyrosine phosphorylation occurs at Tyr72/84/96/178/189. Human BLNK is 456 amino acids (aa) in length and it contains a Pro-rich region (aa 98‑260) and one SH2 domain (aa 346‑453). More than 10 potential splice variants have been detected and among these are four which show single in-frame deletions between aa 203‑225, 366‑417, 146‑417 and 211‑456, respectively. Over aa 226‑456, human BLNK shares 87% and 83% aa identity with canine and mouse BLNK, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.