BLMH/Bleomycin Hydrolase Overexpression Lysate

Images

 
Western Blot: BLMH/Bleomycin Hydrolase Overexpression Lysate [NBL1-07990] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BLMH.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

BLMH/Bleomycin Hydrolase Overexpression Lysate Summary

Description

BLMH/Bleomycin Hydrolase Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200393

Accession#: NM_000386

Protein Tag: C-MYC/DDK
Gene
BLMH

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
52.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BLMH/Bleomycin Hydrolase Overexpression Lysate

  • BHBMHBLM hydrolase
  • bleomycin hydrolase
  • BLMH
  • BMH
  • EC 3.4.22.40

Background

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for BLMH/Bleomycin Hydrolase Lysate (NBL1-07990) (0)

There are no publications for BLMH/Bleomycin Hydrolase Lysate (NBL1-07990).
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Bioinformatics

Gene Symbol BLMH