BLAME/SLAMF8 Antibody (250014) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: Flow
• Clone: 250014
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

BLAME/SLAMF8 Antibody (250014) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BLAME/SLAMF8
  Ala23-Asp233
  Accession # Q9P0V8
• Specificity: Detects human BLAME/SLAMF8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SLAMF7 or rhSLAMF6 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLAMF8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BLAME/SLAMF8 Antibody (250014) [Alexa Fluor® 700]

• B Lymphocyte Activator Macrophage Expressed
• BCM-Like Membrane Protein
• BLAME
• B-Lymphocyte Activator Macrophage Expressed
• CD353 Antigen
• CD353
• SBBI42
• SLAM Family Member 8
• SLAMF8

Background

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19%‑26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).

1. McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
3. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLAMF8
• Uniprot:
  • Q9P0V8()