BIN3 Antibody - BSA Free

Western Blot: BIN3 Antibody [NBP3-35560] - Western blot analysis of various lysates using BIN3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BIN3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BIN3 Antibody - BSA Free (NBP3-35560) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human BIN3 (NP_061158.1).
  
  Sequence:
  MSWIPFKIGQPKKQIVPKTVERDFEREYGKLQQLEEQTRRLQKDMKKSTDADLAMSKSAVKISLDLLSNPLCEQDQDLLNMVTALDTAMKRMDAFNQEKVNQIQKTVIEPLKKFGSVFPSLNMAVKRREQALQDYRRLQAKVEKYEEKEKTGPVLAKLHQAREELRPVREDFEAKNRQLLEEMPRFYGSRLDYFQPSFESLIRAQVVYYSEMHKIFGDLSHQLDQPGHSDEQRERENEAKLSELRALSIVADD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BIN3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:200 - 1:2000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for BIN3 Antibody - BSA Free

• bridging integrator 3
• MGC14978

Background

BIN3, also known as Bridging integrator 3, is a 253 amino acid protein that plays a role in cell membrane shape and structure through interaction with GTPases, and members of the BAR domain protein family have been shown to be involved in processes such as intracellular transport and endocytosis. This protein is currently being studied for research on the following diseases and disorders, ornithosis, pancreatic cancer, and pancreatitis. Interactions with the BIN3 protein have been shown to involve DNMBP, GRPEL1, VDAC3, POR, and RAD23B, CAP2B, and ERH in cell cycle, actin filament organization, cytokinesis, protein localization, and barrier septum assembly pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BIN3