MEPCE Antibody

Images

 
Western Blot: MEPCE Antibody [NB100-93420] - HEK293 overexpressing MEPCE and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: MEPCE Antibody [NB100-93420] - Analysis in human Testis shows staining of the spermatids.
Western Blot: MEPCE Antibody [NB100-93420] - (1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MEPCE Antibody Summary

Immunogen
Peptide with sequence KQQRKFQYGNYCK corresponding to internal region according to NP_062552.2.
Epitope
KQQRKFQYGNYCK
Specificity
Western blot: Preliminary testing was unsuccessful on Human for this particular batch.
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MEPCE
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in rat testis lysates (calculated MW of 74.4 kDa band according to human NP_062552.2 and 75.3 kDa band according to rat XP_222048.4). In transfected HEK293 transiently expressing human MEPCE a band of approx. 75 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human testis shows staining of the spermatids.
Control
MEPCE Overexpression Lysate
Publications
Read Publication using NB100-93420.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MEPCE Antibody

  • BCDIN37SK snRNA methylphosphate capping enzyme
  • bicoid-interacting 3, homolog
  • Bin3 homolog
  • bin3, bicoid-interacting 3, homolog (Drosophila)
  • EC 2.1.1.-
  • FLJ20257
  • methylphosphate capping enzyme

Background

MEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MEPCE Antibody (NB100-93420)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MEPCE
Entrez
Uniprot