MEPCE Antibody

Western Blot: MEPCE Antibody [NB100-93420] - HEK293 overexpressing MEPCE and probed with (mock transfection in first lane).

Immunohistochemistry-Paraffin: MEPCE Antibody [NB100-93420] - Analysis in human Testis shows staining of the spermatids.

Western Blot: MEPCE Antibody [NB100-93420] - (1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Rt, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MEPCE Antibody Summary

• Description: Novus Biologicals Goat MEPCE Antibody (NB100-93420) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-MEPCE Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence KQQRKFQYGNYCK corresponding to internal region according to NP_062552.2.
• Epitope: KQQRKFQYGNYCK
• Specificity: Western blot: Preliminary testing was unsuccessful on Human for this particular batch.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MEPCE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in rat testis lysates (calculated MW of 74.4 kDa band according to human NP_062552.2 and 75.3 kDa band according to rat XP_222048.4). In transfected HEK293 transiently expressing human MEPCE a band of approx. 75 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human testis shows staining of the spermatids.
• Control:

  MEPCE Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MEPCE Antibody

• BCDIN37SK snRNA methylphosphate capping enzyme
• bicoid-interacting 3, homolog
• Bin3 homolog
• bin3, bicoid-interacting 3, homolog (Drosophila)
• EC 2.1.1.-
• FLJ20257
• methylphosphate capping enzyme

Background

MEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MEPCE Antibody (NB100-93420)(1)

Publication using NB100-93420

• Jeronimo C, Forget D, Bouchard A et al. Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Mol Cell 2007-07-20 [PMID: 17643375]

Bioinformatics

• Gene Symbol: MEPCE
• Entrez:
  • 56257(Human)
  • 231803(Mouse)
  • 304361(Rat)
• Uniprot:
  • NP_062552.2(Human)