Biliverdin Reductase B/BLVRB Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

Biliverdin Reductase B/BLVRB Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Biliverdin Reductase B/BLVRB
  Ala2-Gln206, predicted
  Accession # P30043
• Specificity: Detects human, mouse, and rat Biliverdin Reductase B/BLVRB in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Biliverdin Reductase B/BLVRB Antibody [Alexa Fluor® 405]

• biliverdin reductase B (flavin reductase (NADPH))
• Biliverdin Reductase B
• Biliverdin-IX beta-reductase
• BLVRB
• BVRB
• BVR-B
• EC 1.3.1.24
• EC 1.5.1.30
• flavin reductase (NADPH)
• flavin reductase
• FLR
• FLRBVRB
• FR
• GHBP
• Green heme-binding protein
• MGC117413
• NADPH-dependent diaphorase
• NADPH-flavin reductase
• SDR43U1
• short chain dehydrogenase/reductase family 43U, member 1

Background

Clearance of heme in mammals is a two-step process starting with conversion of heme to biliverdin by heme oxygenase, followed by reduction of biliverdin to bilirubin by bilivredin reductase. Biliverdin IX b reductase (BLVRB) converts the  beta  isomer of biliverdin IX to bilirubin IX b, which constitutes 87% of total bilirubin in fetal bile. Therefore BLVRB is especially important for fetal heme metabolism and clearance (1). BLVRB is a cytoplasmic enzyme expressed at high levels in erythrocytes and liver, but is present in other tissues (2). The enzyme is identical to flavin reductase, which is an oxidoreductase that catalyses the NADPH-dependent reduction for a variety of flavins, such as riboflavin, FAD or FMN and met‑hemoglobin (3, 4). BLVRB is structurally distinct from BLVRA. In contrast to BLVRA, which prefers the biliverdin alpha  isomer but could also use the  beta  isomer as substrate, BLVRB is specific for the beta  isomer (5, 6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.